Samuel Gaulden was the son of John Gaulding and Anne Stuart. He lived in St. Peter's Parish, Upper Precinct, New Kent County Virginia. There are also references to him living or at least owning land in Bedford County Virginia and .
Samuel Goulden's [sic] will, dated 06 Oct 1785 in Campbell County, Virginia, mentions children Archibald, William, Kesiah, Ann E., Lucy and Patty. 
Much discussion about Samuel being a son of John Gaulding and Mary Anne Stuart based on her age at the time of his birth. With a birthdate of approximately 1680 she would have been at least 50 at the time of this birth.
↑ 1790 Census of Virginia, 4 white, 1 dwelling, 1 other building, 4 persons total
↑ Virginia State Land Office, Patent E, p.409 "200 Acres granted to Samuel Gaulding in Bedford County Virginia, Signed, Thomas Jefferson"
↑ "Virginia State Land Office">Virginia State Land Office, "To Samuel Gaulding, 1780, in Bedford County Virginia, 200 Acres on both sides of of the north fork of Little Whipping Creek."
↑ William M. Clemens. Virginia Wills Before 1799: A Complete Abstract Register of All Names Mentioned in Over Six Hundred Recorded Wills : Copied from the Court House Records of Amherst, Bedford, Campbell, Loudoun, Prince William and Rockbridge Counties. Pompton Lakes, N.J: Biblio Co, 1924. Print.
Daughters of the American Revolution, DAR Genealogical Research Databases, database online, (http://www.dar.org/ : accessed 09 Dec 2016), "Record of Samuel Gaulden", Ancestor # A204851.
Private tree on FTDNA
The Gaulden, Gauldin, Gaulding, Family History -- A Seven Hundred Year Study, Volume One, p. 24, By Dr. Charles H. Gaulden, Self Published, April, 1999.
"Blankenship ancestors : Agee, Dunn Johnston, Jones, Ligon, Moseley, Perdue, White", Gayle King Blankenship, Book 65, 1995, self published Virginia. Geneagophers
This Profile was created by a GEDCOM import. WikiTree Profile Gaulding-26 by Tim Philips through the import of Phillips Family Tree_2014-01-11_2014-01-11.ged on Jan 11, 2014.
Source: S00054 Title: Ancestry Family Trees Publication: Name: Online publication - Provo, UT, USA: The Generations Network. Original data: Family Tree files submitted by Ancestry members.; Repository: #R00001 NOTE This information comes from 1 or more individual Ancestry Family Tree files. This source citation points you to a current version of those files. Note: The owners of these tree files may have removed or changed information since this source citation was created.
It may be possible to confirm family relationships with Samuel by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
Y-chromosome DNA test-takers in his direct paternal line on WikiTree: