↑ "Connecticut Marriages, 1630-1997", database, FamilySearch (https://familysearch.org/ark:/61903/1:1:F7GD-JYS : 31 March 2016), James Gladwin and Laura Knowles, 1824. NOTE: American Sentinel And Witness, Issue: 7 Apr 1824, pg32
Research Notes
There are several entries in familysearch.org giving a marriage date of April 13, 1824, without citing a source for that info. Given the publication date of the source cited in the bio, it is likely this date is a date of publication of a marriage notice in an unnamed newspaper.
Familysearch.org also has a marriage notice for James R. Gladwin and Phebe A. Knowles on July 23, 1846, published in the Hartford Courant, Issue: 8 Aug 1846, pg1066, "Connecticut Marriages, 1630-1997", database, FamilySearch (https://familysearch.org/ark:/61903/1:1:F7GD-JYH : 31 March 2016), James R. Gladwin and Phebe A. Knowles, 1846. The coincidence of surnames of bride and groom may indicate some relationship
Acknowledgement
WikiTree profile Gladwin-1 created through the import of Timerson Family Tree May162011.ged on Jun 6, 2011 by Brad Timerson.
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DNA Connections
It may be possible to confirm family relationships with James by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with James: