James Hacker was married twice. First to Anne Clark who must have passed away between 1855 and 1856. Children born to them were
Daniel born 1850 in Indiana
Melvina born 1851 in Indiana
Nancy A. born 1855 in Indiana
After Anne's death, James married Elizabeth Jane Methvin and had several more children
Mary born 1860 in Indiana
John born 1861 in Indiana
Ella born 1862 in Indiana
Selena born 1867 in Missouri
Rena born 1870 in Missouri
Cora born 1876 in Missouri
Sara born 1871 in Missouri
Samuel born 1875 in Missouri
Maggie born 1876 in Missouri
Sources
Marriage to Anne Clark "Indiana Marriages, 1811-2019," database with images, FamilySearch (https://www.familysearch.org/ark:/61903/1:1:XXYG-KLD : 3 August 2022), James Hacker and Ann Clarke, 19 Sep 1848; citing Bartholomew, Indiana, United States, Marriage License, Indiana Commission on Public Records, Indianapolis; FHL microfilm 004200265.
Marriage to Elizabeth Jane Methvin "Iowa Marriages, 1809-1992", database, FamilySearch (https://familysearch.org/ark:/61903/1:1:XVSP-ZQK : 13 January 2020), Elizabeth Jane Methvin in entry for James Hacker, 1856.
1850 census "United States Census, 1850", database with images, FamilySearch (https://www.familysearch.org/ark:/61903/1:1:MHVN-Z7K : Thu Oct 05 13:20:11 UTC 2023), Entry for James Hacker and Anna Hacker, 1850.
1860 census "United States Census, 1860", database with images, FamilySearch (https://www.familysearch.org/ark:/61903/1:1:M4FC-QRK : Fri Oct 06 01:31:26 UTC 2023), Entry for James Hacker and Elizabeth J Hacker, 1860.
1870 census "United States Census, 1870", database with images, FamilySearch (https://www.familysearch.org/ark:/61903/1:1:M467-HLY : Thu Oct 05 01:56:23 UTC 2023), Entry for James Hacker and Elizabeth Hacker, 1870.
1880 census "United States Census, 1880", database with images, FamilySearch (https://www.familysearch.org/ark:/61903/1:1:M6X3-8YW : Wed Oct 04 08:25:39 UTC 2023), Entry for James Hacker and Elisbeth Hacker, 1880.
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DNA Connections
It may be possible to confirm family relationships with James by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with James: