"United States Census, 1850," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:MCJQ-2BL : 12 April 2016), Jas A Hightower in household of Thos Hightower, Claiborne parish, part of, Claiborne, Louisiana, United States; citing family 180, NARA microfilm publication M432 (Washington, D.C.: National Archives and Records Administration, n.d.).
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DNA Connections
It may be possible to confirm family relationships with James by comparing test results with other carriers of his ancestors' Y-chromosome or mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with James: