Anderson, Robert Charles. Great Migration: Immigrants to New England, 1634-1635, Volume III, G–H. (Boston: NEHGS, 2003): pp.397-401, biograghy of Nicholas Holt (father of Nicholas Holt, Jr.) including list of his children.
A Genealogical History of the Holt Family in the United States: More Particularly the Descendants of Nicholas Holt of Newbury and Andover, Mass., 1634-1644, and of WIlliam Holt of New Haven, Conn. by Daniel S. Durrie. Albany: J. Munsell, 78 State Street, 1864, pp. 12 & 15 [2]
Holt Association of America. The first three generations of Holts in America. (Newburgh, NY: Holt Association of America, 1930), pp.151-183. HathiTrust.org [3]
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DNA Connections
It may be possible to confirm family relationships with Nicholas by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Nicholas: