John Hopkin was born say 1550, probably in Hampshire, England. There was a person by the name of Hopkins mentioned in Upper Clatford, Hampshire in a deposition dated 07 February 1579, as having been present there 26 years or so before, and perhaps a relative of John. John Hopkins first is recorded in Upper Clatford when he married in 1574. By 1586, he has removed to Winchester where he is mentioned in the lay subsidies in 1586. 
John married first Agnes Borrowe on 28 Oct 1574 at Upper Clatford, Hampshire, England, and had two children, William and Alice. John married secondly Elizabeth WillIams on 28 Jul 1579 at Upper Clatford and had two children, Susanna and Stephen.
He probably died not long before 04 October 1593 in Winchester, when administration on his estate was granted to widow Elizabeth, William Hopkins posting the bond. William may have been John Hopkins' brother or other close relative, and not likely his son who would have been to young at age 18 to sign the bond.
Removed Stephen Hopkins as John's father. A Rev. Stephen Hopkins did exist but had no relationship to Stephen Hopkins of the Mayflower. John Hopkins was Stephen's father but a brother of John named Nicholas Hopkins married to Mary Poole is invented.
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