Ann was born about 1857. She is the daughter of William Hughes and Jane.
Sources
England Births and Christenings, 1538-1975," database, FamilySearch (https://familysearch.org/ark:/61903/1:1:NLHY-CV8 : 11 February 2018, Ann Hughes, 29 Mar 1857); citing Tarvin, Cheshire, England, index based upon data collected by the Genealogical Society of Utah, Salt Lake City; FHL microfilm 1,850,125.
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DNA Connections
It may be possible to confirm family relationships with Ann by comparing test results with other carriers of her ancestors' mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Ann: