James was a carpenter and farmer born in Attleboro, Massachusetts, on 2 June 1716. He married Mary Day there in 1738. Their children born in Attleboro were Olive in 1741, Amos in 1743, twins Phillis and Thankful in 1745, Levi in 1747, Mary in 1749, Amaziah in 1750, James in 1752, and Elkanah who died young.
↑ Jillson, David "Genealogy of the Gillson and Jillson Family" E.L.Freeman & Company (1876) pp.135-136
Massachusetts Births and Christenings, 1639-1915. Birth of James Jillson on 2 Jun 1716 at Attleboro, Bristol, Massachusetts Bay to James Jillson and Susanah. 
Family Search ["Massachusetts, Town Clerk, Vital and Town Records, 1626-2001," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:QG1K-MX3W : 25 February 2020), James Jelson and Mary Day, 17 Feb 1738; citing Marriage, Town of Attleborough, Plymouth, British Colonial America, Massachusetts Secretary of the Commonwealth, Boston; FHL microfilm 007009342.]
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It may be possible to confirm family relationships with James by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with James: