Isaac II was the son of Isaac and his second wife Lydia (Jones) Keen/Keene
He married Bethia Toby/Tobey, daughter of Eleazer & Margaret (Fish) Toby/Tobey, on 26 Aug 1764 at Sandwich, Barnstable, MA - their children were Eleanor, Isaac III & James
Sources
MA Town & Vital Records, 1620-1988
www.genealogyofnewengland.com
Mayflower Families Through Five Generations, Silver Books, Volume Eighteen, Part 2, The Descendants of Richard Warren Through His Children Mary, Anna, and Elizabeth, Published 2011, Page 122
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DNA Connections
It may be possible to confirm family relationships with Isaac by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Isaac: