Nothing is known by me at this time besides her name and that she was married to John Layman, from Botetourt, VA.
Sources
Layman Family Bible pages
"Places Near the Mountains" "Botetourt and Roanoke Counties Virginia page 137"
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DNA Connections
It may be possible to confirm family relationships with Mary by comparing test results with other carriers of her mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Mary: