Francis, son of Francis Knollys and Catherine Carey was born on Monday 14 August 1553.  He was their 6th son.  He attended Magdalen College, Oxford and then was a student of Gray's Inn in 1565. 
From the age of about 22 Sir Francis was MP for Oxford and he served in the Parliament for about 20 years. 
During his youth, he sought to raise his fortunes by piracy and adventure. He was part of an early expedition to colonise North America.  Eight years later, in 1586, he served as a Rear-Admiral under Sir Francis Drake on an "official" privateering campaign. His next adventure was to serve with his brother in law Robert Dudley, Lord Leicester in the Netherlands. He was knighted by Leicester at Flushing in Dec 1587. During the Armada invasion he served the Crown as as commissioner for musters and colonel of the militia for Hertfordshire.
On 21 Dec 1588 he married Lettice Barrett of Hanham. Their marriage took place, by license, at All Hallows London Wall.  It apopears that during their married life they lived in a number of properties in Berkshire. From Burkes: "He inherited under his father's will the manor of Battle, and other estates in the vicinity of Reading, and was styled of Reading Abbey."
Sir Francis had his will drawn up on 6 Dec 1646.  He made bequests to:
daughter Lady Vachell
daughter Lady Knollys, wife of my late son Sir Francis Knollys
grandchild Lettice Woodward
grandchild Richard Knollys
grandchild Elizabeth Knollys, daughter of the Lady Eleanor Knollys.
daughter Elizabeth Hammond
her son Robert Hammond.
Sir Francis requested burial in the church of St Lawrence, Reading, Berkshire. This took place on 17 Mar 1648.  The register recorded him as Sir Francis Knollys, jun.
↑ Varlow, Sally Sir Francis Knollys's Latin dictionary: new evidence for Katherine Carey Historical Research, Volume 80, Issue 209, August 2007, Pages 315–323,Oxford Academic
↑ Ancestry.com. England & Wales, Prerogative Court of Canterbury Wills, 1384-1858 [database on-line]. Provo, UT, USA: Ancestry.com Operations, Inc., 2013. The National Archives; Kew, England; Prerogative Court of Canterbury and Related Probate Jurisdictions: Will Registers; Class: PROB 11; Piece: 204 Ancestry Record 5111 #812548
↑ Coates, Charles The History and Antiquities of Reading 1802 pg. 230 Google Books
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It may be possible to confirm family relationships with Francis by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
Y-chromosome DNA test-takers in his direct paternal line on WikiTree: