Anne Lee was born on June 18, 1839 in Fairfax Parish, Arlington, Virginia, United States. Her parents were Robert Lee and Mary Custis.
She died on October 20, 1862 in Warrenton, Warren, North Carolina, United States
From Find A Grave:
Anne, or Annie as she was called, was the second daughter and third child of Confederate General Robert E. Lee and his wife, Mary Anna Custis Lee and she was the only child of the seven Lee children to die before her father. Black-haired and pretty, Annie was the least outgoing of the four Lee girls and she was extremely attached to her father, as he was to her. She lost the sight in one eye due to an accident with a pair of scissors but she managed, despite continuing ill health, to enjoy teaching slave children and she was once captured by Federal troops during the Civil War, along with her mother and her sister, Mildred. Upon Mrs. Lee's request, an exchange was made and she and her daughters were allowed (by the Union General McClellan) to pass unmolested back into Confederate territory where General Lee was personally waiting for them with open arms. Annie developed typhoid fever in the autumn of 1862 and, despite desperate measures by family members, she died. General Lee never recovered from the grief of losing his "Annie".
Annie was originally buried near Warrenton, North Carolina but, in 1994, her remains were moved to lie with her family members in the Lee Chapel and Museum in Lexington, Virginia.
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It may be possible to confirm family relationships with Annie by comparing test results with other carriers of her ancestors' mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Annie: