"United States Census, 1850," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:MX77-N6D : 12 April 2016), Margs Sprout in household of Jacob Sprout, Jackson, Seneca, Ohio, United States; citing family 71, NARA microfilm publication M432 (Washington, D.C.: National Archives and Records Administration, n.d.).
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It may be possible to confirm family relationships with Margaret by comparing test results with other carriers of her mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Margaret: