"United States Census, 1850," index and images, FamilySearch (https://familysearch.org/pal:/MM9.1.1/MXQQ-XVF : accessed 12 May 2014), Nicholas Longworth in household of Joseph Longworth, Spencer, Hamilton, Ohio, United States; citing family 255, NARA microfilm publication M432.
"United States Census, 1860," index, FamilySearch (https://familysearch.org/pal:/MM9.1.1/MCLL-H2G : accessed 12 May 2014), Nicholas Longworth in household of Joseph Longworth, Spencer Township, Hamilton, Ohio, United States; citing "1860 U.S. Federal Census - Population," Fold3.com; p. 165, household ID 1297, NARA microfilm publication M653; FHL microfilm 803979.
"United States Census, 1870," index and images, FamilySearch (https://familysearch.org/pal:/MM9.1.1/M6VK-NSJ : accessed 12 May 2014), Nicholas Longworth in household of Joseph Longworth, Ohio, United States; citing p. 42, family 329, NARA microfilm publication M593, FHL microfilm 000552707.
 The Cabells and Their Kin: A Memorial Volume of History, Biography, and Genealogy Alexander Brown January 1, 1895 Houghton, Mifflin & Company: Parents, Marriage, Wife, Children, Birth, Death page 404, 405
Judge Nicholas Longworth, II (1844-1890) on Find A Grave: Memorial #78985775 Retrieved 17 December 2017.
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It may be possible to confirm family relationships with Nicholas by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.