The identification of John Madison and Isabella Minor Todd as great grandparents of President James Madison comes from a hand-written pedigree chart ascribed to James Madison himself.
A digital image of the original pedigree is available through the Library of Congress. It may have been donated to the Library of Congress by Mrs. Mary Madison McGuire of Washington City, who had the original in her possession at the time it was published in the W&MQ.
NOTE: That his wife [[Minor-671|Isabella] was born a Todd has been disputed. See her profile for discussion.
Regarding birth location, King and Queen was not formed until 1691 out of New Kent County.
"The Bulk of the influential men in Virginia at the time, were composed of merchants from English cities and ship captains. To this class belonged the first John Madison, who in a patent dated Sept. 1682, calls himself 'Ship Carpenter. He had the following grants: 1658, 300 acres in Westmoreland Co.; 1653, 600 acres on North side of Mattapony River... In Sept. 1682: of 'Saint Stephen Parish, New Kent.' He died before Apr. 16, 1683, since at that time John Madison, Jr., patented--part thereof 300 acres, 'formerly granted to John Madison, Sr., deceased'. This John Madison [Jr] was sheriff of King and Queen co. in 1704. There is a deed on record in King William Co., dated 1706, from John Madison, of St. Stephens Parish, K.& Q Co., and ISABELLA his wife. There is of King William Co. (cut from K.& Q., in 1701). John Madison & Isabella (Todd) his wife, had issue. (1) Ambrose (g.f. of Pres.) (2) Capt. John".
"The Andrea file [what is this?], p. 23, A&B, shows John Madison, (Jr) m. ISABELLA MINOR TODD. to the right he has listed "Elizabeth?" Ambrose (the ? is his). He erred in assuming that her mother was an Ambrose, and that she had been married to a Minor. Proof of this is found in Emperor Charlemagne's Descendants, v. II, Gen.Pub. Co., Inc., p. 178:
"Isabella Todd, youngest child, b. in VA 1670; m. John Madison II, was dau of Ann Gousuch, b. at Walkern, Herts, Eng., Mar. 13, 1638/9, d. at 'Toddsbury,' Gloucester Co., VA, between May 24, 1694 and Nov. 10, 1697; m.(1) CAPT. THOMAS TODD, b. at Denton Co, Durham, Eng., d. at sea, 1676; He came to VA Feb. 22, 1653; m.pr. 30 May 1677, Ann Gorsuch, dau. of Rev. John & and Ann Lovelace Gorsuch, D.D. Ann dau of Sir William Lovelace, Knt. & Anne Barnes; The trace goes on to Charlemagne and of course beyond."
* Source: U.S. and International Marriage Records, 1560-1900
Source: "Emperor Charlemagne's Descendants, v. II, Gen.Pub. Co., Inc., p. 178."
“The Madison Family Tree,” Founders Online, National Archives (http://founders.archives.gov/documents/Madison/01-01-02-0063-0001 [last update: 2016-03-28]). Source: The Papers of James Madison, vol. 1, 16 March 1751 – 16 December 1779, ed. William T. Hutchinson and William M. E. Rachal. Chicago: The University of Chicago Press, 1962, pp. 4–6.
It may be possible to confirm family relationships with John by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share DNA with John: