It may be possible to confirm family relationships by comparing test results with James or other carriers of his ancestors' Y-chromosome or mitochondrial DNA.
Y-chromosome DNA test-takers in his direct paternal line on WikiTree:
Family Tree DNA Y-DNA Test 67 markers, haplogroup R-U106, FTDNA kit #54221
Mitochondrial DNA test-takers in the direct maternal line:
Family Tree DNA mtDNA Test HVR1 and HVR2, haplogroup H, FTDNA kit #54221