Peter Massie was born August 29, 1718 in St. Peter's Parish, New Kent, Virginia 
There is evidence in New Kent County, Virginia public birth records that an Ann Massie is the mother of Sylvanus Massie born 08 Apr 1738. The spouse of Ann Massie, and father of Sylvanus is indicated as being Peter Massie.  Ann's (b 1722) maiden name is unproved but said to be Raymond.  An Ann Massie is listed in the St Peter's parish records and we have a public record that indicates an Ann Massie is the mother of Sylvanus Massie, son of Peter Massie.
The record contains the following:
Other information in the record of Silvanus Massie from Virginia, Births and Christenings
Name Silvanus Massie
Christening Date 26 May 1738
Christening Place ST PETER'S PARISH,NEW KENT,VIRGINIA
Birth Date 08 Apr 1738
Father's Name Peter Massie
Mother's Name Ann
CITING THIS RECORD:
"Virginia, Births and Christenings, 1853-1917," database, FamilySearch (https://familysearch.org/ark:/61903/1:1:VRR4-MH5 : accessed 9 October 2015), Ann in entry for Silvanus Massie, 08 Apr 1738; citing , reference ; FHL microfilm 6,048,679.
baptized May 21, Silvanus son of Peter and Ann Massie, born April 8, 
This information below from a 1906 issue of William And Mary College Quarterly, also provides additional evidence that Peter Massie was married to an Ann.
File transcribed and submitted to the USGenWeb Archives by:
Edward Reynolds email@example.com
William And Mary College Quarterly. Vol 15. No 1. Pages 24-36 July 1906.
"REGISTER OF ST. JAMES NORTHAM PARISH, GOOCHLAND COUNT, 1756, Oct. (Sylvanus Massie and Hannah Raglin, both in this parish.)
Sylvanus MASSIE - b. Apr. 8, 1738, New Kent Co., VA; bap. May 26, 1738, St. Peter's Parish, New Kent Co., VA; d. after 1786, Campbell Co., VA. Son of Peter Massie and Ann. In Buckingham Co., VA in 1774, but in Campbell Co., VA in 1781 when he furnished supplies to the Continental Army. Married first Oct. 3, 1757, St. James Northam Parish, Goochland Co., VA, to Hannah RAGLAND by Rev. William Douglas. Married second after 1772, Mary ANDERSON HUDSON, daughter of Hugh ANDERSON, widow of Simon HUDSON, Sr., and mother of Frances 'Fannie' HUDSON MASSIE, wife of Thomas B. MASSIE. 
↑ "Virginia Births and Christenings, 1584-1917," database, FamilySearch (https://familysearch.org/ark:/61903/1:1:VRRW-K2Q : 10 February 2018), Charles Massie in entry for Peter Massie, 29 Aug 1718; citing ST PETER'S PARISH,NEW KENT,VIRGINIA, reference ; FHL microfilm 6,048,679.
1684-1786 - Chamberlayne, C.G., ed.. The Vestry Book and Register of St. Peter's Parish New Kent and James City Counties, Virginia 1684-1786. Richmond, Va.: Library Board, 1937. Available at FHL Collection; digital versions at Ancestry ($); VAGenWeb - free.
Source: S21 Author: Smith, Paulette Title: New Kent County, Virginia Land Tax Records, 1782 Publication: Name: Online publication - Provo, UT, USA: Ancestry.com Operations Inc, 1999.; Repository: #R1
It may be possible to confirm family relationships with Peter by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share DNA with Peter: