↑ "Virginia Marriages, 1785-1940," database, FamilySearch (https://familysearch.org/pal:/MM9.1.1/XRZ8-KVQ : 5 December 2014), James W. Mayo and Roxy Ann Layne, 05 Feb 1812; citing Goochland County, Virginia, reference 114; FHL microfilm 31,650.
↑James Mayo of Virginia by Mrs. Bert Harter in The Virginia Genealogist (article)
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DNA Connections
It may be possible to confirm family relationships with James by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with James: