Had a daughter Agnes who died at birth 31 Jan 1910.
"Pennsylvania, Philadelphia City Death Certificates, 1803-1915," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:J692-2QV : 8 March 2018), John H. Mcfarlane in entry for Agnes Mcfarlane, 31 Jan 1910; citing cn 3231, Philadelphia City Archives and Historical Society of Pennsylvania, Philadelphia; FHL microfilm 1,405,121.
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It may be possible to confirm family relationships with John by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with John: