According to "The Descendants of William McPheeters"; Samuel married Margaret Searight and together they had 7 children.
Only one is known,
Samuel McPheeters is the son of William McPheeters and Rebecca Thompson. He was born in ABOUT 1740 in Chester Co., PA. He married Margaret Seawright (probably in Augusta Co., VA).
Section: The descendants of William McPheeters
Source InformationAncestry.com. The McPheeters family [database on-line]. Provo, UT: Ancestry.com Operations Inc, 2005.
Original data: Rice, Helen McPheeters.. The McPheeters family. Winter Park, Fla.: unknown, 1956. 
Created by: LSP Added: 29 May 2011
Find A Grave Memorial 70589222
Source Hide citation Find A Grave, database and images (https://www.findagrave.com : accessed 01 December 2019), memorial page for Samuel McPheeters (1740–1830), Find A Grave Memorial no. 70589222, citing New Providence Presbyterian Cemetery, Surgoinsville, Hawkins County, Tennessee, USA ; Maintained by LSP (contributor 46860931) .
User ID: 9EA7375E-E84D-48A1-8735-6CF3C3BFAE10
Record ID Number: MH:IF11808
Place: Chester County, Pennsylvania
User ID: EEFFB6E0-9B31-4F89-A8A4-5518EAC0C143
Record ID Number: MH:IF11807
Place: Hawkins County
Record ID Number =
Record ID Number: MH:I442
User ID: 56B8D31E-5721-4712-B0C7-216D5465C9D6
UPD 18 MAY 2012 16:35:20 GMT-6
User ID: BFFD9E86-08CB-4C5C-9BFC-499122CB6D37
Record ID Number: MH:IF11809
Place: New Providence Presbyterian Cem, Surgonisville, Tenn
WikiTree profile McPheeters-59 created through the import of Wiki Tree.GED on Jun 30, 2012 by Dale Smith. See the Changes page for the details of edits by Dale and others.
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It may be possible to confirm family relationships with Samuel by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share DNA with Samuel: