John Adolphus Nelles was born on February 14, 1844, in Hamilton, Ontario, Canada. He married Margaret H. Cuthbertson on November 16, 1865 in York, Haldiman, Haldimand-Norfolk, Ontario, Canada, and they had one child. He then married Betsy Leitch on February 19, 1874 in Elgin, Ontario, Canada and they had two children. He third married Priscilla C Pearson (1854-1945) on May 7, 1879 in Caledonia, Haldimand, Ontario, Canada and they had four children together. He died on October 2, 1918, in Stratford, Ontario, Canada, at the age of 74.
"Ontario, County Marriage Registers, 1858-1869," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:Q2T1-BL3X : 31 March 2017), John Adolphus Nelles and Margt H Cuthbertson, 16 Nov 1865; citing Haldimand, Ontario, Canada, Archives of Ontario, Toronto; FHL microfilm 1,030,057.
"Ontario Deaths, 1869-1937 and Overseas Deaths, 1939-1947," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:JKWD-BRH : 11 December 2014), John Adolphus Nelles, 02 Oct 1918; citing Stratford, Perth, Ontario, 218, Archives of Ontario, Toronto; FHL microfilm 1,862,884.
Nellis-Nelles Immigrants From the Palatinate 1710, Ontario, Canada, Vol. 1 published in 1997 by The Nellis and Nelles Family Associations and the Herkimer County Historical Society, #3576, page 496
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It may be possible to confirm family relationships with John by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
Y-chromosome DNA test-takers in his direct paternal line on WikiTree: