He appears to have been born in either Virginia or North Carolina. He was a completely different individual from the Nathaniel Nichols who spent his entire life in New England, who was born at about the same time, but died in 1785.
He died between 16 Sep and 31 Oct 1755, per his will and probate papers.
Minerva Bone Basset, in her book, refers to Nathaniel having married Anna Booth. Perhaps that was before his marriage to Mary, since Mary was still alive at the time of his death and is named in his estate papers as his wife [See Bassett, Minerva Bone - _Some Webster County, Kentucky, Families_ (Baltimore, MD: Gateway Press - 1983) - p3 .]
Three legitimate children are named in his will: Josiah, William, and Anne (married to George Stanley). Josiah is almost definitely the product of Nathaniel's marriage to Mary. The mother of William and Anne, both apparently older than Josiah, could be Mary.
Nicholas, Nathaniel. Bertie Co. (NC) will made on 17 Sep 1755 -- viewed on Ancestry.com .
Probate note, re: Nicholas, Nathaniel. Bertie Co. (NC) Court Oct 1755 - viewed on Ancestry.com .
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It may be possible to confirm family relationships with Nathaniel by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share DNA with Nathaniel: