"United States Census, 1850," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:M44J-7GH : 12 April 2016), Barbara Osburn in household of Jacob Osburn, Broadtop, Bedford, Pennsylvania, United States; citing family 66, NARA microfilm publication M432 (Washington, D.C.: National Archives and Records Administration, n.d.).
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It may be possible to confirm family relationships with Barbara by comparing test results with other carriers of her mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Barbara:
It shows links to her husband, parents, 5 children and 11 siblings. It states that while she died in MO she was buried in IA. Please view this memorial. You have Jacob with two wives with the same dates but different places of death and different spellings of their maiden names. I believe McClain-226 and McLain-65 are the same person. Since you manage both profiles and the original source was your Kayla1800.ged, you need to make this merge!
http://findagrave.com/cgi-bin
And Memorial#31386694
It shows links to her husband, parents, 5 children and 11 siblings. It states that while she died in MO she was buried in IA. Please view this memorial. You have Jacob with two wives with the same dates but different places of death and different spellings of their maiden names. I believe McClain-226 and McLain-65 are the same person. Since you manage both profiles and the original source was your Kayla1800.ged, you need to make this merge!