James Posselthwaite was married to Hannah (no further information). His father was John Posselthwaite (no further info), and his mother was named Alice (no further info).
Sources
Email from my mother's second cousin.
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DNA Connections
It may be possible to confirm family relationships with James by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with James: