"United States Census, 1850," database with images, FamilySearch (https://www.familysearch.org/ark:/61903/1:1:M65X-F16 : 20 December 2020), John A Rush, Meade, Kentucky, United States; citing family , NARA microfilm publication (Washington, D.C.: National Archives and Records Administration, n.d.).
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It may be possible to confirm family relationships with John by comparing test results with other carriers of his ancestors' Y-chromosome or mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with John: