"United States Census, 1850," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:MZ46-BVL : 12 April 2016), Margaret Scott in household of Abigail Scott, Jackson, Henry, Iowa, United States; citing family 1432, NARA microfilm publication M432 (Washington, D.C.: National Archives and Records Administration, n.d.).
"Iowa, County Marriages, 1838-1934," database, FamilySearch (https://familysearch.org/ark:/61903/1:1:XJC4-998 : 3 November 2017), George M. Carlock and Margaret S. Bailey, 13 Jun 1861, Mahaska, Iowa, United States; citing reference vol 3 p 85, county courthouses, Iowa; FHL microfilm 985,381.
"United States Census, 1880," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:M6XQ-12Z : 7 September 2017), Margaret S Carlock in household of George M Carlock, Jefferson, Harrison, Missouri, United States; citing enumeration district ED 297, sheet 124C, NARA microfilm publication T9 (Washington D.C.: National Archives and Records Administration, n.d.), roll 0689; FHL microfilm 1,254,689.
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It may be possible to confirm family relationships with Margaret by comparing test results with other carriers of her ancestors' mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Margaret: