Paternal relationship is confirmed through Y-chromosome DNA testing. A direct male descendant of Samuel Shirley FTDNA Kit# 66774 and a direct male descendant of Samuel's third cousin, Neville Shirley, FTDNA kit #78112, match at a Genetic Distance of 0 on 37 markers thereby confirming their direct paternal lines back to their MRCA John Shirley as reported in FamilyTreeDNA SHIRLEY/SHERLEY/SHURLEY/ DNA Project. This indicates over a 95% probability of a common ancestor within eight generations.
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