Mary was born in 1842. She was the daughter of Francis Southworth and Nancy Hunter Southworth. She passed away after 1860.
Sources
1860 U.S. Federal Population Census, New York, Franklin County, Town of Bangor, Page 121, July 27, 1860
A Genealogy of the Southworths (Southards) Descendants of Constant Southworth
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DNA Connections
It may be possible to confirm family relationships with Mary by comparing test results with other carriers of her ancestors' mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Mary: