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Y-DNA Haplogroup R-M335

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R1B1B (R-M335) R1b1b is defined by the presence of SNP marker M335. This haplogroup was created by the 2008 reorganisation of nomenclature and should not be confused with R1b1b2, which was previously called R1b1c. Its position in relation to the much more populous sub-clade R1b1b is uncertain.[1] The M335 marker was first published in 2004, when one example was discovered in Turkey, which was classified at that time as R1b4.[1]

R1b1b (M335) has only been found in Anatolia. (Modern Turkey) [2]

R1b1b (R-PH155) The other secondary branch of R1b is R-PH155 (R1b1b), which is extremely rare and defined by the presence of PH155.[53] ISOGG (2017) cites two primary branches: R-M335 (R1b1b1) and R-PH200 (R1b1b2).

Another defining SNP of R1b1b, M335, was first documented in 2004, when an example was discovered in Turkey, which was classified at that time as R1b4.[23]

Other examples of R-PH155 have been found in Bahrain, Bhutan, Tajikistan and Xinjiang.

The early R1b cattle herders would have split in at least three groups. One branch (M335) remained in Anatolia, but judging from its extreme rarity today wasn't very successful, perhaps due to the heavy competition with other Neolithic populations in Anatolia, or to the scarcity of pastures in this mountainous environment.[3]

Message from my first M335 Match


I recently received my Big-Y test results from Family Tree DNA and you are my only match. Apparently R-M335 (aka R1b1b1) is a very rare haplogroup.

We differ by 17 variants. So assuming 144 years per mutation and assuming an equal number of mutations in both our lines, it puts the minimum time to our most recent common ancestor at about 1200 years or so. So, no point in trying to cousin-match :o)

Like you, I have traced my paternal line back to the late 18th century, i.e., just before the start of Italian Civil records.

Here is what I know about the origins of my paternal line: My paternal ancestors (along with many other Albanians) emigrated from Albania to Italy sometime in the second half of the 15th century. Obviously, I cannot prove that with a paper trail, but a Damis family was one of the Albanian families that settled in the 15th century in the area that eventually became the town of Lungro in Calabria (from secondary sources re the history of Lungro). All of my known paternal paper-trail ancestors (except for my father) were born in Lungro. Lungro is located on the side of a mountain and up until recently was pretty isolated. Most of the residents of Lungro are of Albanian descent and (up until recently) speak a dialect of Albanian in addition to Italian. They have retained many Albanian customs. My grandparents spoke a mixture of Albanian and Italian. Family lore says that we are Albanian.

So, my question to you is, as far as you know, is your paternal line of Albanian origin?

Thanks in advance for answering that, and I promise not to pester you again.

Best regards, Bob Damis


  1. http://www.gutenberg.us/articles/eng/haplogroup_r1b_(y-dna)
  2. http://www.eupedia.com/europe/Haplogroup_R1b_Y-DNA.shtml
  3. http://www.eupedia.com/europe/Haplogroup_R1b_Y-DNA.shtml

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