Margaret married Marcum Lott "Mark" Manning I - parents of three sons, including Marcum II & Lewis
She died in Clarke Co., MS - death date & burial info Unknown
Sources
1830-1850 US Federal Censuses.
Find a Grave, database and images (https://www.findagrave.com/memorial/162574565/margaret-landers-manning: accessed 10 April 2023), memorial page for Margaret Landers Spaulding Manning (1776–1860), Find a Grave Memorial ID 162574565; Burial Details Unknown; Maintained by K (contributor 46499361).
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DNA Connections
It may be possible to confirm family relationships with Margaret by comparing test results with other carriers of her mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Margaret: