"United States Census, 1940," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:K7YH-5QG : accessed 1 January 2018), Trixie Spence in household of Willie Spence, Magisterial District 5, Martin, Kentucky, United States; citing enumeration district (ED) 80-7, sheet 11A, line 31, family 159, Sixteenth Census of the United States, 1940, NARA digital publication T627. Records of the Bureau of the Census, 1790 - 2007, RG 29. Washington, D.C.: National Archives and Records Administration, 2012, roll 1339.
It may be possible to confirm family relationships with Trixie by comparing test results with other carriers of her ancestors' mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share DNA with Trixie: