"United States Census, 1850," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:MD9X-2DX : 9 November 2014), Ambrose Spencer, Lee, Berkshire, Massachusetts, United States; citing family 77, NARA microfilm publication M432 (Washington, D.C.: National Archives and Records Administration, n.d.).
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It may be possible to confirm family relationships with Ambrose by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
Y-chromosome DNA test-takers in his direct paternal line on WikiTree: