A Francis Spencer son of John was baptised at Baldock, Hertfordshire, three miles from Stotfold where the Spencer family lived, in Sep 1584. That a Francis was the son of John is certain through the wills of his uncle Richard Spencer who named Daniel son of his brother John, and Francis' own will in which he names his uncle Richard Spencer and his brother Daniel. Francis was the son of John Spencer.
His wife was named as Margaret in his will, and if she was the mother of all his children the marriage, untraced, was before 1613.
He made a will dated 7 Apr 1636, proved 24 Oct 1636. He describes himself as citizen and brewer of London, of the parish of St. Giles without Cripplegate. He mentions his estate in Hitchin, co. Hertford, and names his wife Margaret and the four children below; and appoints as overseers his uncle Richard Spencer, citizen and haberdasher of London, and his brother Daniel Spencer, citizen and grocer of London. Issue: Thomas, Susan, Elizabeth, Agnes. The three daughters were all under the age of 21. All 4 children appear to have been baptised at Hitchin, the earliest in Jun 1613.
He died before 24 Oct 1636, when his will was proved.
↑ "England Births and Christenings, 1538-1975," database, FamilySearch (https://familysearch.org/ark:/61903/1:1:NB6M-SX5 : 11 February 2018, Francis Spenser, Sep 1584); citing , index based upon data collected by the Genealogical Society of Utah, Salt Lake City; FHL microfilm 991,305.
↑ The National Archives; Kew, England; Prerogative Court of Canterbury and Related Probate Jurisdictions: Will Registers; Class: PROB 11; Piece: 172 
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It may be possible to confirm family relationships with Francis by comparing test results with other carriers of his ancestors' Y-chromosome or mitochondrial DNA.
Y-chromosome DNA test-takers in his direct paternal line on WikiTree: