Nathaniel Spencer was born 05 Dec 1704 Hartford, Hartford, Connecticut,  son of Samuel Spencer and Deborah Beckley. He married first Abigail Hurlbut 1 Mar 1732/33 at Middletown, Middlesex, Connecticut and had the following known children:
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It may be possible to confirm family relationships with Nathaniel by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
Y-chromosome DNA test-takers in his direct paternal line on WikiTree: