"United States Census, 1840," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:XHYT-7HX : 24 August 2015), John Sprout, Seneca, Ohio, United States; citing p. 242, NARA microfilm publication M704, (Washington D.C.: National Archives and Records Administration, n.d.), roll 426; FHL microfilm 20,176.
"United States Census, 1850," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:MX77-N6K : 12 April 2016), John Sprout in household of Jacob Sprout, Jackson, Seneca, Ohio, United States; citing family 71, NARA microfilm publication M432 (Washington, D.C.: National Archives and Records Administration, n.d.).
Year: 1790; Census Place: Hopewell, Newton, Tyborn, and Westpensboro, Cumberland, Pennsylvania; Series: M637; Roll: 8; Page: 15; Image: 534; Family History Library Film: 0568148
1830; Census Place: Oxford, Guernsey, Ohio; Series: M19; Roll: 131; Page: 368; Family History Library Film: 0337942
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It may be possible to confirm family relationships with John by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with John: