"United States Census, 1850," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:MX77-N6F : 12 April 2016), David Sprout in household of Jacob Sprout, Jackson, Seneca, Ohio, United States; citing family 71, NARA microfilm publication M432 (Washington, D.C.: National Archives and Records Administration, n.d.).
"United States Civil War Soldiers Index, 1861-1865," database, FamilySearch (https://familysearch.org/ark:/61903/1:1:FS6C-1PT : 4 December 2014), David Alexander Sprout, Private, Company E, 49th Regiment, Ohio Infantry, Union; citing NARA microfilm publication M552 (Washington D.C.: National Archives and Records Administration, n.d.), roll 103; FHL microfilm 882,316.
"Ohio Death Index, 1908-1932, 1938-1944, and 1958-2007," database, FamilySearch (https://familysearch.org/ark:/61903/1:1:VK13-STM : 8 December 2014), David A Sprout, 25 Jan 1927; from "Ohio, Deaths, 1908-1932, 1938-2007," database and images, Ancestry (http://www.ancestry.com : 2010); citing vol. , certificate number , Ohio Historical Society, Columbus; Ohio Department of Health, State Vital Statistics Unit, Columbus.
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It may be possible to confirm family relationships with David by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with David: