Hon Edward Smith-Stanley, later 12th Earl of Derby
Maj Hon Thomas Smith-Stanley (b. 1753; d. 1779)
Lady Elizabeth Smith-Stanley (d. 13 Apr 1796), mar. 28 Jul 1778 Rev Sir Thomas Horton, 3rd Bt., and had issue
Lady Lucy Smith-Stanley (d. 1833), mar. 25 Apr 1772 Col Rev Geoffrey Hornby DL, of Scale Hall, co. Lancaster, High Sheriff of Lancashire 1774, Rector of Winwick, co. Lancaster 1782-1812 (b. 1750; d. 31 Jul 1812), only son of Edmund Hornby, of Poulton-in-Fylde and Scale Hall, co. Lancaster, by his wife Margaret Winckley, 2nd dau. of John Winckley, of Preston, co. Lancaster, and had issue
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It may be possible to confirm family relationships with James by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
Y-chromosome DNA test-takers in his direct paternal line on WikiTree: