Margaret was born in 1825. It inicates that she is of English descent. 
Ancestry.ca marriage certificate
Name: Margaret Stanley
Spouse: Oliver Johnston
Event: Mariage (Marriage)
Marriage Year: 1846
Marriage Location: Percé and Cape Cove, Québec (Quebec)
Place of Worship or Institution: Anglican Church
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It may be possible to confirm family relationships with Margaret by comparing test results with other carriers of her mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share DNA with Margaret: