The 1880 census for Robert Stuart's household shows the folowing people:
Robert Stuart, self (47, born Tennessee)
Nancy Stuart, wife (35, born Tennessee)
son Marion Stuart (18, born Tennessee)
son James A Stuart (16, born Tennessee)
daughter Ursley M Stuart (15, born Tennessee)
son Stephen Stuart (12, born Tennessee)
son John Stuart (11, born Tennessee)
daughter Ermine Stuart (6, born Tennessee)
daughter Mary A Stuart (5, born Tennessee)
son Henry Stuart (2, born Tennessee)
boarder Gemima Delk (92, born North Carolina)
↑ "United States Census, 1880," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:MD7Q-RHW : 15 July 2017), Robert Stuart, District 8, Fentress, Tennessee, United States; citing enumeration district ED 39, sheet 557B, NARA microfilm publication T9 (Washington, D.C.: National Archives and Records Administration, n.d.), FHL microfilm 1,255,254. Image (accessed 3 October 2020).
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It may be possible to confirm family relationships with Unknown by comparing test results with other carriers of her ancestors' mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Unknown: