Mary Stuart, was the third daughter of James VI/I King of Scotland and England, and his wife Anne of Denmark, and their first child born once James had succeeded as King of England.
She was born at Greenwich, most likely on 8/18 April 1605, and was baptised at Greenwich Chapel, 5 May 1605. Her godparents were her maternal uncle, Ulric, Duke of Holstein; her father's cousin, Lady Arabella Stuart and the Countess of Northumberland.
The Lady Mary died young on 16 September 1607 at Stanwell Park, Middlesex, the seat of Sir Thomas Knyvett, where she was living.. She died aged 2 years, 5 months and 8 days, according to her memorial in Westminster Abbey, where she was buried on 23 September 1607.
↑ Howes, Edmond, The annales, or generall chronicle of England,
begun first by maister Iohn Stow, and after him continued and augmented with matters forreyne, and domestique, anncient and moderne, vnto the ende of his present yeere 1614; London: T. Adams, 1615. Digital image, HathiTrusthttps://catalog.hathitrust.org/Record/100217153 : accessed 30 December 2019.
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It may be possible to confirm family relationships with Mary by comparing test results with other carriers of her ancestors' mitochondrial DNA.
Mitochondrial DNA test-takers in the direct maternal line: