Charles Stuart (22 October 1660 – 5 May 1661) was the first of four sons and eight children born from the marriage between James, Duke of York (later James II of England & VII of Scotland) and his first wife, Anne Hyde. He was styled Duke of Cambridge, but never formally created so, because of his early death. Charles died at the Palace of Whitehall on 5 May 1661  and was buried in Westminster Abbey.
↑ The Diary of Samuel Pepys 6 May 1661 "I hear tonight that the Duke of York's son is this day dead, which I believe will please every body; and I hear that the Duke and his Lady themselves are not much troubled at it."
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It may be possible to confirm family relationships with Charles by comparing test results with other carriers of his ancestors' Y-chromosome or mitochondrial DNA.
Y-chromosome DNA test-takers in his direct paternal line on WikiTree: