According to Dr. Gaulden's book "Gaulden..a seven hundred year history", Alexander Gaulden "son of Anne Stuart and John Gaulden of New Kent Co died Dec. 1752, and James Stuart was given custody of one of his minor children. This would support the assumption that his mother's maiden name was Stuart. Perhaps James Stuart was Anne's brother, thus the court ruled in favor of the great-uncle (James Stuart) rather than the uncle (John Gaulden II)"
The source given for this information is "The Court of Dyer and Terminer in Williamsburg, VA, Library, E69626.8 975.51 N46 SA24, and in "Prince Edward County Court Records, Book 1, p. 115 , March Ct. 1757" as well as in the "Barksdale Family History and Geneology"
"Ann wife of Jno. Gauling dy’d Octo. 27 (1735).
The Gaulden, Gauldin, Gaulding, Family History -- A Seven Hundred Year Study, Volume One, P. 24, By Dr. Charles H. Gaulden, Self Published, April, 1999.
St. Peters Parish Church Register, p.120, New Kent County, VA.
Source: S1730862093 Repository: #R1730539867 Title: Ancestry Family Trees Publication: Online publication - Provo, UT, USA: Ancestry.com. Original data: Family Tree files submitted by Ancestry members. Note: This information comes from 1 or more individual Ancestry Family Tree files. This source citation points you to a current version of those files. Note: The owners of these tree files may have removed or changed information since this source citation was created. Page: Ancestry Family Trees Note: Data: Text: 
It may be possible to confirm family relationships with Mary by comparing test results with other carriers of her mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share DNA with Mary: