Ann is not listed as a daughter of Christopher Threlkeld and his wife Sussannah. That couple did have a daughter Mary Ann who married Cornelius Vaughan and they had a son John who had two daughters that married into the Bohon family: Sarah m. Benjamin Bohon, and Judith m. Walter Bohon
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DNA Connections
It may be possible to confirm family relationships with Ann by comparing test results with other carriers of her mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Ann:
Threlkeld-167 and Unknown-357178 appear to represent the same person because: It appears that Threlkeld-167 has gone through some levels of misidentification and was linked to Benjamin, Jr who was married to Sarah Threlkeld rather than being linked to Benjamin, Sr , married to Ann (MNU).