"United States Census, 1860," database, FamilySearch (https://familysearch.org/ark:/61903/1:1:MCG6-3BZ : accessed 23 November 2015), Chas A Twining, Township Of Russia, Lorain, Ohio, United States; from "1860 U.S. Federal Census - Population," database, Fold3.com (http://www.fold3.com : n.d.); citing p. 20, household ID 140, NARA microfilm publication M653 (Washington, D.C.: National Archives and Records Administration, n.d.); FHL microfilm 805,002.
"United States Census, 1870," database with images, FamilySearch (https://beta.familysearch.org/ark:/61903/1:1:M6LM-FW6 : accessed 24 November 2015), Charles Twining, Ohio, United States; citing p. 1, family 3, NARA microfilm publication M593 (Washington D.C.: National Archives and Records Administration, n.d.); FHL microfilm 552,734.
"United States Census, 1900," database with images, FamilySearch (https://beta.familysearch.org/ark:/61903/1:1:MMDF-3TZ : accessed 24 November 2015), Charles A Twining, Camden & Henrietta Townships, Lorain, Ohio, United States; citing sheet 312B, family 306, NARA microfilm publication T623 (Washington, D.C.: National Archives and Records Administration, n.d.); FHL microfilm 1,241,295.
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It may be possible to confirm family relationships with Charles by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage (beta) of DNA with Charles: