Daughters of the American Revolution, DAR Genealogical Research Databases, database online, (http://www.dar.org/ : accessed 19 Oct 2019), "Record of Robert Tyler", Ancestor # A117704.
Brogan, Hugh and Charles Mosley, American Presidential Families, New York: Macmillan Publishing Company (1993), 657.
Cheney, Lynne, Blue Skies, No Fences: A Memoir of Childhood and Family, New York: Threshold Editions (2009), Chart on pages 308-309. Lynne Cheney in her book "Blue Skies, No Fences" gives Margaret's maiden name as Tyler
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It may be possible to confirm family relationships with Margaret by comparing test results with other carriers of her mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage (beta) of DNA with Margaret: