Charlotte was born in 1816. She passed away in 1871.
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Sources
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Find a Grave, database and images (accessed 18 December 2020), memorial page for Charlotte Hardaway Watson Ames (10 Sep 1816–2 Dec 1871), Find A Grave: Memorial #7066561, citing Wanda Cemetery, Wanda, Newton County, Missouri, USA; maintained by Silvia (contributor 47129208), headstone photo.
"Alabama County Marriages, 1809-1950," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:QKZ3-H357 : 19 February 2021), John L. Ames and Charlotte H. Watson, 11 Sep 1834; citing Greene, Alabama, United States, County Probate Courts, Alabama; FHL microfilm 1,290,852.
"Texas Deaths, 1890-1976," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:K3WQ-K7M : 20 February 2021), Watson in entry for J H Ames, 20 Aug 1913; citing certificate number 18945, State Registrar Office, Austin; FHL microfilm 2,050,996.
May be born on September 10, 1816 according to Edward L. Ames' Bible.
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DNA Connections
It may be possible to confirm family relationships with Charlotte by comparing test results with other carriers of her mitochondrial DNA.
However, there are no known mtDNA test-takers in her direct maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Charlotte: