According to his headstone in Wethersfield, he was age 49 at death.
His estate was probated in Hartford in 1776 (packet #5819) -- his inventory of L2501 included land in Wethersfield, Colchester, Glastonbury, Windsor and Harwinton.
Sources
"Connecticut Births and Christenings, 1649-1906," database, FamilySearch (https://familysearch.org/ark:/61903/1:1:F74Z-LZ1 : accessed 2 November 2015), James Welles in entry for James Welles, 23 Mar 1766; citing ; FHL microfilm unknown.
Mayflower Families through five generations, Volume Eleven, Edward Doty published by the General Society of Mayflower Descendants 1996 , page 78
Vital Records of Connecticut "Electronic," Source Information: Batch number: Dates Source Call No. Type Printout Call No. Type 7028707 - 0538644 Film NONE Sheet: 55 * https://www.findagrave.com/memorial/132991664
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DNA Connections
It may be possible to confirm family relationships with James by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with James:
Wells-17855 and Wells-16174 appear to represent the same person because: I created Wells-17855 and later found the same person already existed in the system.