She was originally attached as the daughter of Ebenezer Holmes. His daughter, mentioned in his estate distribution in 1763 was deceased and unmarried or married to a Holmes.
Sources
↑ "Massachusetts, Town Clerk, Vital and Town Records, 1626-2001," database with images, FamilySearch (https://www.familysearch.org/ark:/61903/1:1:Q29L-G764 : 20 May 2022), Betty Holmes, 16 Jan 1734; citing Birth, Plymouth, Plymouth, Massachusetts Bay Colony, British Colonial America, Massachusetts Secretary of the Commonwealth, Boston; FHL microfilm 004934186.
Esther Littleford Woodsworth-Barnes, Mayflower Families through Five Generations, Vol 16, Part 1, The Descendants of John Alden, General Society of Mayflower Descendants, 2002. "The Silver Book" Page 268
Mayflower Families Through Five Generations, Silver Books, Volume Eighteen, Part 2, The Descendants of Richard Warren Through His Children Mary, Anna, and Elizabeth, Published 2011, Page 68
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DNA Connections
It may be possible to confirm family relationships with Betty by comparing test results with other carriers of her mitochondrial DNA.
Mitochondrial DNA test-takers in the direct maternal line: