"United States Census, 1850," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:MHV8-TRM : 4 April 2020), John Chastine, Vernon, Washington, Indiana, United States; citing family 307, NARA microfilm publication M432 (Washington, D.C.: National Archives and Records Administration, n.d.).
"United States Census, 1880," database with images, FamilySearch (https://familysearch.org/ark:/61903/1:1:MHSR-ZRG : 19 August 2017), John Chastain, Vernon, Washington, Indiana, United States; citing enumeration district ED 184, sheet 521D, NARA microfilm publication T9 (Washington, D.C.: National Archives and Records Administration, n.d.), FHL microfilm 1,254,321.
Find a Grave, database and images (accessed 05 September 2020), memorial page for John R Chastain Sr. (1 Oct 1828–1 Sep 1896), Find A Grave: Memorial #46064048, citing Lost River Cemetery, Campbellsburg, Washington County, Indiana, USA ; Maintained by Goddess (contributor 46995829) .
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DNA Connections
It may be possible to confirm family relationships with John by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with John:
~0.78%Mark Curtis :
Family Tree DNA Family Finder, GEDmatch QY9130370[compare], FTDNA kit #785430
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Autosomal DNA Test, GEDmatch A266440[compare], yourDNAportal MAR348ca4e4