Samuel was born about 1738. He married Elizabeth sometime before 1760 when they had their first known child, who was baptised in Wattisfield, Suffolk, England.
Sources
Children:
England, Select Births and Christenings, 1538-1975, FHL Film Number 0825410 (RG4 1863): Elizabeth Colby, daughter of Samuel Colby and Elizabeth, baptised 26 May 1760, Wattisfield, Suffolk, England.
"England Births and Christenings, 1538-1975", database, FamilySearch (https://www.familysearch.org/ark:/61903/1:1:JM2W-WBF (sourced 14 Oct 2023): Mary Colby daughter of Samuel Colby and Elizabeth, baptised 7 Jun 1767
England, Select Births and Christenings, 1538-1975, FHL Film Number 0825410 (RG4 1863): "England Births and Christenings, 1538-1975", database, FamilySearch (https://www.familysearch.org/ark:/61903/1:1:JM2W-49Z (accessed 14 Oct 2023): Elisha Colby, son of Samuel Colby and Elizabeth, baptised 11 Aug 1770 in Wattisfield, Suffolk, England
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DNA Connections
It may be possible to confirm family relationships with Samuel by comparing test results with other carriers of his Y-chromosome or his mother's mitochondrial DNA.
However, there are no known yDNA or mtDNA test-takers in his direct paternal or maternal line.
It is likely that these autosomal DNA test-takers will share some percentage of DNA with Samuel: